BiP is a protein in the lumen of the ER. In order to evaluate DiOC6(3)
staining, double labeling with DiOC6(3) and anti-BiP was done. CV-1
cells were fixed in glutaraldehyde, stained with DiOC6(3) then
photographed. The cells were then put into cold methanol and processed
for immunofluorescence using anti-BiP. A comparison of the staining
showed that the network stained by DiOC6(3) is also stained by anti-BiP.
DiOC6(3) also stains other organelles, including mitochondria. This is
shown in the following two image movie
have not figured out how to preserve image quality in animations, so the
image quality here is not as good as it should be). Scale bar = 10
microns. Arrow in DiOC6(3) image points to a mitochondria that is not
labeled in the anti-Bip image.